Membrane components were prepared at 5 107 cells/ml using 1% NP-40 in PBS with 1 mM AEBSF, 1 M pepstatin A, 10 M E-64, 10 M bestatin, 100 M leupeptin, and 2 g/ml aprotinin (lysis buffer)
Membrane components were prepared at 5 107 cells/ml using 1% NP-40 in PBS with 1 mM AEBSF, 1 M pepstatin A, 10 M E-64, 10 M bestatin, 100 M leupeptin,…