After removal of the cellular debris by centrifugation at 4000?rpm for 30?min at 4?C, the cleared lysate was loaded onto a Ni2+-NTA Fast Begin Package column (Qiagen), as well as the enzyme was purified under local conditions following manufacturers process
After removal of the cellular debris by centrifugation at 4000?rpm for 30?min at 4?C, the cleared lysate was loaded onto a Ni2+-NTA Fast Begin Package column (Qiagen), as well as…