Liver X Receptors

The compound was purified by silica gel column chromatography (hexanes to 10% ethyl acetate in hexanes)

The compound was purified by silica gel column chromatography (hexanes to 10% ethyl acetate in hexanes). C was treated with potassium bis(trimethylsilyl)amide (KHMDS, 0.5 M, 1.5 mL, 0.75 mmol) and…

Continue ReadingThe compound was purified by silica gel column chromatography (hexanes to 10% ethyl acetate in hexanes)

This was followed by incubation with salmon sperm DNA/protein A agarose slurry for 1?h, then samples were sequentially washed with low salt buffer, high salt buffer, LiCl buffer, and twice with TE buffer

This was followed by incubation with salmon sperm DNA/protein A agarose slurry for 1?h, then samples were sequentially washed with low salt buffer, high salt buffer, LiCl buffer, and twice…

Continue ReadingThis was followed by incubation with salmon sperm DNA/protein A agarose slurry for 1?h, then samples were sequentially washed with low salt buffer, high salt buffer, LiCl buffer, and twice with TE buffer