Another medical aspect of the maturational delay in IgA is usually that it may be associated in some children with non-IgE-mediated food allergies . In this study, total serum IgE in children with autism was higher than in healthy children, but the difference did not reach the significance threshold. of CD19/CD23 was found in children diagnosed with autism than in the control group (36.82 6.72% vs. 18.20 3.95%; 0.02). No correlation between the quantity of CD23-positive cells and serum IgE levels was observed. Conclusions A delicate shift of serum immunoglobulins consisting of low-normal IgA and B cell activation expressed by an increase of CD23-positive cells may characterize children with regressive autism aged 3-6 years old. = 18/= 6). The inclusion criteria for the control group were the same as criteria 2 and 3 for the autism group listed above. Participants were excluded if they experienced an acute contamination or chronic diseases, experienced taken medications, vitamins, or supplements, or if dietary intervention or option therapy was applied. Children for each case-control pair were recruited in the same allergic season and the time interval of the blood sample collection from both (the case and the control) was no longer than 4 weeks. None of the control children were recruited from families with a history of ASD. All study participants were Caucasian. This study Rabbit Polyclonal to Glucagon protocol followed ethical guidelines and was approved by the Bioethics Committee of the Medical University or college of Bialystok. Informed consent was obtained from the parents prior to participation. Laboratory analysis Blood samples were collected between 7 am and 9 am. Immediately after centrifugation the sera were aliquoted Darunavir Ethanolate (Prezista) and frozen at C80C until assayed for immunoglobulin levels. Serum IgG, IgM, and IgA concentrations were measured using the nephelometric technique (Siemens nephelometer BN II system) with a detection range of 0.06-8.00 g/l for IgA, 1.4-45.0 g/l for IgG, 0.05-6.40 g/l for IgM. The reference range of serum immunoglobulins for children aged 3 to 6 years in the children’s hospital laboratory are: IgA 0.36-2.40 g/l, IgG 5.0-13.2 g/l, IgM 0.46-1.75 g/l, IgE 0-85 IU/ml. Serum IgA levels less than 7 mg/dl (0.07 g/l) were accepted as diagnostic of selective IgA deficiency and levels at least 2 SD (standard deviations) below normal for age were diagnostic of partial IgA deficiency . Total IgE and gluten-specific IgG (f79) were detected using the UniCAP fluoroenzymeimmunoassay (Pharmacia, Sweden) according to the manufacturer’s instructions. The reference range of IgE in the laboratory for children 3-4 years of age is usually 0-33 IU/ml, and for children 5 years of age is usually 0-85 IU/ml. Serum hsCRP and serum tumour necrosis factor (TNF-) were measured using a commercially available ELISA Darunavir Ethanolate (Prezista) kit (Quantikine High Sensitivity Human by R&D Systems, Minneapolis, Minn., USA) and according to the manufacturer’s instructions. Assessment of peripheral blood morphology. For the assessment of the leukogram component, 2 ml of venous blood was obtained in blood collection tubes made Darunavir Ethanolate (Prezista) up of EDTA. A blood count analysis was performed using automated haematology analyzers (SYSMEX XT 2000i, Japan). Leukocyte count was expressed in G/L and lymphocytes were expressed as percentages (%). Assessment of the lymphocyte subpopulation in peripheral blood. Cytometric analysis was performed around the blood which remained after the morphological assessment mentioned above. The following monoclonal antibodies were added in 10 l amounts to 100 l of full blood (Beckman Coulter): CD3-PC5, CD4-FITC, CD4-PC5/CD25-FITC, CD8-PE, CD19-PC5, CD19-PC5/CD23-PE. Statistical analysis Student’s 0.05 were considered statistically significant. MedCalc was used for the ROC analysis, GraphPad Prism 5.0 was used for comparative analysis, and a logistic regression model was created using Statistica 9.0 software. Results Table I shows the baseline characteristics of.