Immunohistochemistry was performed using biotin-conjugated appropriate secondary antibodies (Jackson ImmunoResearch Laboratories, Western Grove, PA), horseradish peroxidase-linked avidin-biotin complex reagents (Dako, Glostrup, Denmark), and 3,3-diaminobenzidine (Sigma-Aldrich) while immunodetection substrate, while previously described (34), and then counterstained with thionine blue
Immunohistochemistry was performed using biotin-conjugated appropriate secondary antibodies (Jackson ImmunoResearch Laboratories, Western Grove, PA), horseradish peroxidase-linked avidin-biotin complex reagents (Dako, Glostrup, Denmark), and 3,3-diaminobenzidine (Sigma-Aldrich) while immunodetection substrate, while previously…